Sentence examples for mediated insertion of from inspiring English sources
In these studies [ 5, 6, 7], increased dSir2 expression was mediated from the endogenous dSir2 locus through use of a P-element mediated insertion of the UAS sequence upstream of dSir2 to generate an "EP" line [ 9], allowing induced expression of dSir2 from its endogenous locus when the GAL4 protein is present.
Instead, since RCAS is a retrovirus we decided to analyze RCAS mediated insertion of PDGF-B and HRG cDNA in genomic DNA isolated from tumor tissue.
The UAS-dGPHR, UAS-dGPHR::HA, UAS-huGPHR, UAS-huGPHR::HA and UAS-hamsGPHR flies were obtained by P element mediated insertion of pUAST constructs containing full length cDNA coding for dGPHR, dGPHR::HA, huGPHR, huGPHR::HA and hamsGPHR, respectively (molecular details available upon request).
After we have demonstrated the twin ribozyme mediated insertion of additional nucleotides into a predefined position of a suitable substrate RNA, we here show that a similar type of twin ribozyme is also capable of mediating the opposite reaction: the site-specific removal of nucleotides.
Alternate approaches for correcting the majority of X-CGD mutations were assessed, involving TALEN- or Cas9-mediated insertion of CYBB minigenes at exon 1 or 2 of the CYBB locus.
Nested PCR was performed to test whether HR-mediated insertion of the R2HR donor fragment had occurred.
pCFJ151 contains homology arms that direct transposase-mediated insertion of intervening sequence into the ttTi5606 Mos1 site on Chromosome II.
The two approaches described above were used to obtain piggyBac-mediated insertion of the gfp-expression cassette into the P. berghei genome.
All five promoters were assayed by recombineering-mediated insertion of gfp immediately after each of the unique initiation codons (WBIDs Expr9763/9764/9768/9780/9782).
This library can be extended by the Red-mediated insertion of φ80- attB sites into new points on MG1655 chromosome with deleted native φ80- attB.
A HA-Pur-alpha transgenic fly line was produced by phiC31 integrase-mediated insertion of the pUAST-attB vector in the strain 24749, on the third chromosome.
