Sentence examples for mediated injection of from inspiring English sources

Gene-gun mediated injection of this experimental TBEV vaccine into adult mice is shown to yield a neutralizing and protective immune response (4 ).

Another way by which plant-beneficial Pseudomonas strains may suppress local host immune responses is via T3SS-mediated injection of effector proteins.

Immunostaining analysis and the measurement of enzymatic activity showed that the liver was efficiently transduced upon AAV2/8-TBG-mediated injection of the modified and wild-type sulphamidase enzyme (Fig 3A and B).

The significance of focal adhesions in promoting cell elongation has been emphasized by the finding that β1 integrin-mediated injection of CagA is important in the process of cell elongation [ 24].

In contrast, the AAV2/8-TBG-mediated injection of the fully modified sulphamidase in MPS-IIIA mice led to a significant increase in sulphamidase activity levels up to 10 15% greater than the activity measured in the brain of control mice (Fig 4A).

We employed tail-vein-mediated injection of plasmid DNA/lipid complex for kidney-targeted gene transfer, since i) the method itself is non-invasive (it does not require surgical treatment), simple and convenient, and ii) successful gene delivery to the kidney has been reported from several laboratories with this method [ 18- 24].

Each construct was then introduced into PAK-JΔSTY and tested for proper expression under type III inducing condition as well as efficiency of T3SS mediated injection into host cells.

This effect was not tumor mediated because injection of TBHsp70 in tumor-free mice induced an increase of Tregs in the draining lymph nodes as well as inhibition of proliferation of lymph node T cells and an increase in IL-10 production.

More recently, a breast cancer xenotransplantation model demonstrated that a single adenoviral-mediated intra-tumoural injection of a DKK3 expression vector efficiently discontinued tumour growth, with the induction of apoptosis in these cells [ 26].

It is somewhat surprising that we observed lower rates of HDR when injecting gRNAs as DNA since previous studies have reported very high rates of CRISPR/Cas9-mediated NHEJ with injection of in vitro transcribed gRNAs (Bassett et al. 2013; Yu et al. 2013).

These results demonstrate the benefits of SELP 815K for matrix-mediated gene delivery over the injection of free virus and the injection of virus in Poloxamer 407.