These findings suggested that in the WT brain, Nova-mediated inclusion of Scn9a E17a was normally used to produce transcripts that are likely degraded by NMD, and that E17a exclusion in the absence of NOVA activity could lead to an increase in steady state mRNA and protein level.
Degradation of BTK in the Ramos cell line appears to be proteasome-mediated as inclusion of the well-characterized proteasome inhibitor Bortezomib results in a partial rescue of the observed degradation.
Considerations should be given to metabolism-mediated toxic effects (inclusion of metabolic competence).
One of the major contributions of dynamic assessment over static assessment is that the inclusion of mediated interaction gives us more information apropos of a learner's abilities After all, two heads are better than one.
Time to DSS 3 in multivariate analysis accounts for the effect of Years 1 and 2 relapses, probably by heralding the progressive course and the effect may have been underestimated by inclusion of relapse-mediated arrival at DSS3.
Cellular attachment to a biomaterial surface may occur through inclusion of integrin-mediated focal adhesion complexes or materials that enable electrostatic interactions between the surface and the cellular membrane.
Platforms for the development of synthetic chromosomes in plants have been produced in several species using telomere mediated chromosomal truncation with the simultaneous inclusion of sites that facilitate further additions to the newly generated minichromosome.
During whole-cell recording, GABAAR-mediated responses were pharmacologically isolated by inclusion of the ionotropic glutamate receptor blocker kynurenic acid (2 m m; Sigma) in the recording solution.
The use of heparinase to degrade the heparin present in the sample is one technique to overcome heparin-mediated quantitative PCR inhibition, and the inclusion of a ruminant spike prior to heparinase digestion ensures accurate quantitation.
Isoform 3 (Fig. 2D and Data not shown) could not be amplified, but we reasoned that this may be due to the action of nonsense-mediated decay (NMD) resulting from the inclusion of a premature termination codon (PTC) located in intron 1.
In this work, Man et al. [ 92] developed a series of synthetic sRNAs designed to target 5′ UTRs to trigger RNase E-mediated degradation and showed that the inclusion of natural Hfq-binding sites stabilized the synthetic sRNA in vivo and facilitated gene silencing.
