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Few prior investigations reported the use of electroporation mediated gene transfer to cardiac tissue ex-vivo.
Furthermore, there is a recent report of gene transfer agent mediated gene transfer in the natural populations of Roseobacter [15].
The plasmid was transformed into A. thaliana ecotype Columbia via Agrobacterium- mediated gene transfer (Clough and Bent 1998).
Virus-vector mediated gene transfer in vitro could be another option [26].
Electroporation mediated gene transfer to the beating heart is safe Fifty-eight animals were used in the entire study.
Adenoviral mediated gene transfer results in transient gene expression.
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Electric pulse-mediated gene transfer into FDB muscle was performed as described (DiFranco et al, 2009).
Osbeck and Poncirus trifoliate by Agrobacterium-mediated gene transfer.
You can read the research paper entitled Vector-mediated gene transfer engenders long-lived neutralizing activity and protection against SIV infection in monkeys at Nature.com.com
Agrobacterium-mediated gene transfer is governed by various factors of bacterial, host and environmental origin.
This has encouraged the development of non-viral DNA-mediated gene transfer techniques such as liposome, virosomes, microsphere and nanoparticles.
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