One model revolves around the chaperone-mediated extraction of mitochondrial proteins [ 265].
This suggests that p97-mediated extraction of MOM proteins is unlikely the exclusive cause for PINK1-Parkin-mediated cell death.
These findings, taken together with the strong, selective binding of triethylenetetramine to Cu II) [ 9, 11], are consistent with protection by the drug against any copper toxicity that could otherwise be caused in the body during trientine-mediated extraction of copper into the urine.
Diverse ubiquitin adapters are known to link p97 to its client proteins, but two recent papers on the adapter protein UBXD7, including one by Bandau et al. in BMC Biology, suggest that rather than simply linking p97 to ubiquitylated proteins, this adapter may be essential to coordinate ubiquitylation and p97-mediated extraction of the proteasome substrate.
The biophysical characterization of purified membrane proteins typically requires detergent mediated extraction from native lipid membrane environments.
Much work lies ahead to understand the individual functions of the adapters, how p97 complexes modulate and coordinate events upstream and downstream of p97-mediated extraction and how this is embedded in complex signaling networks.
The feasibility of employing micelle-mediated extraction as an alternative and effective method for the solubilization, purification and/or preconcentration of active ingredients from herbal products is demonstrated for the first time using the root of American ginseng as a model.
A thermoresponsive polymer-mediated extraction was designed for the selective separation of traces metal ions from high purity iron solutions.
This suggests that linking substrate ubiquitylation and p97-mediated extraction may be important for efficient degradation of some substrates.
Figure 1b demonstrates the power of the LCM technique, showing the section images before and after the laser-mediated extraction, as well as the cellular population of the GP3 and GP4 isolated on the LCM caps.
