Sentence examples for mediated extension from inspiring English sources

We therefore determined whether upregulation of autophagy mediated extension of life span by rapamycin.

The following amplification conditions described by Islam et al.[ 23] were used for ctxA, tcpA ET and tcpACL genes: an initial denaturation at 94°C for 5 minutes, followed by a middle step of 40 cycles for 1 minute each at 94°C (denaturation), 56°C (annealing of primer) and 72°C (DNA polymerase– mediated extension) and a final extension step at 72°C for 10 minutes.

Herein, we firstly present a novel electrochemical DNA biosensor based on grafting-to mode of TdTase-mediated extension, in which DNA targets are polymerized in homogeneous solution and then hybridized with DNA probes on BSA-based DNA carrier platform.

In this work, a sensitive SPR-DNA sensor was developed by employing in situ synthesis of copper nanoparticles (CuNPs) templated by poly-T sequences DNA from terminal deoxynucleotidyl transferase (TdT -mediated exTdT -mediatedsynextensionlly with nand-effect deposynergisticallyass relay.

Overexpression of human NNMT in yeast increases rDNA silencing, a phenomenon linked to sirtuin-mediated extension of life span [41].

Because Sanger sequencing relies on molecular separation of the polymerase-mediated extension of the primer, the first ∼20 50 bases 3' of the primer are not discernible.

After purification, telomerase-mediated extension of a nontelomeric primer was measured by quantitative PCR (IP-RQ-TRAP) (Wege et al, 2003; Cohen and Reddel, 2008).

Increased mitochondrial activity plays also a causative role in CR-mediated extension of Drosophila lifespan, since knock-down of either complex I or IV subunits leads to diminished lifespan extension under CR [ 41].

They also imply that in Drosophila, additional factors act alongside dFOXO to produce IIS-dependent responses in body size, fecundity, and oxidative stress resistance and that these phenotypes are not causal in IIS-mediated extension of lifespan.

cDNA was synthesized from DNase-treated total RNA, using a M-MLV reverse transcriptase-mediated extension of random primers, followed by a RT-PCR amplification using different sets of primers (available upon request).

One highly regarded model for telomere length regulation involves a negative-feedback or telomere protein-counting mechanism in which the length of the telomere and the abundance of the associated proteins produce a cis-acting negative feedback signal for telomerase-mediated extension (Marcand et al. 1997; Van Steensel and De Lange 1997).