Sentence examples for mediated excision of from inspiring English sources

The loxP elements were used to allow Cre mediated excision of the cassette in a cell autonomous manner (Figure S3).

In this study, we made use of a strain of mice containing a mutant K-Ras allele (K-RasG12D) that can be conditionally activated by Cre-recombinase mediated excision of a floxed transcription termination (LSL) cassette [16].

The following combinations of the primers were used: to check presence of distal loxP sites LF/LR; to check Cre mediated excision of the locus LF/ER; to check Flp mediated excision of Neomycin cassette EF/ER.

Our mutagenesis system offers an ability to conditionally revert mutagenic insertions into non-mutagenic ones by Cre-recombinase mediated excision of the Gal-VP16 (and UAS:eGFP) sequences.

The source of the circular donor plasmid that contains both attB sites and the GFP cassette is a Flp mediated excision of a transgenic insertion.

This strain expresses the Tomato transgene only upon Cre mediated excision of the floxed-translation Stop sequence in the Rosa26 locus.

The EGFP gene is expressed only after Cre-mediated excision of loxP-flanked stop sequences.

This block in transcription should be relieved by Cre recombinase-mediated excision of the floxneo cassette.

Cre-mediated excision of SRF floxed alleles was detected as previously described [16].

This orientation should enable FLP-mediated excision of the hdhfr-resistance cassette located between the FRT sites.

For detection of Cre-mediated excision of exons 2 10 of p53 (hereafter designated p53Δ2-10), p53int1fwd3int1fwd and p53int10rev were used, yielding a 612 bp product.