Model results showed that longer dispersing plants may have a competitive advantage in their colonization success as compared to the better competitors, especially in the cases of a disturbance-mediated creation of gaps in the landscape.
We additionally tested the possibility that polyadenylation-mediated creation of termination codons occurs preferentially in certain functional gene categories.
Our analyses of published genomic data from Blastocystis sp. subtype 7 revealed that polyadenylation-mediated creation of termination codons occurs in approximately 15% of all nuclear genes.
Based on the foregoing estimate of approximately 15%, we assume that approximately 900 of the 6,000 annotated genes in this Blastocystis subtype rely on polyadenylation-mediated creation of termination codons.
C to U RNA editing-mediated creation of the AUG initiation codon from the original ACG codon is a common phenomenon observed in case of chloroplast genome-derived transcripts.
Genes exhibiting polyadenylation-mediated creation of termination codons in the Blastocystis sp. subtype 1 were identified in a draft genome assembly obtained from 454 and Illumina reads (Gentekaki E, Curtis B, Roger AJ, unpublished data) using EST data available for this isolate (Stechmann et al. 2008).
Based on the known functions of Anx2, we propose that the most likely mechanism behind our results is Anx2-mediated creation and/or stabilization of the PtdIns(4,5 P2-containing lipid raft membrane domains from which the virus preferentially buds [16]–[21].
While N. benthamiana provides a more amenable model system to elucidate the molecular mechanisms underlying SP/HrpZ-mediated BNYVV resistance, the creation of stable transformants of sugar beet, the natural host of BNYVV, might prove an efficient strategy to combat rhizomania.
The remaining mutations, one nonsense mutation and two single nucleotide deletions, are expected to result in nonsense-mediated decay through the creation of a premature stop codon.
In contrast to Michael additions, thiol ene reactions are a radically mediated step growth polymerization, which requires creation of initial radicals either thermally or photochemically.
We have found retrovirus-mediated expression to be the fastest method for creation of stable clones and it produces the most consistent and long-lasting expression.
