Model results showed that longer dispersing plants may have a competitive advantage in their colonization success as compared to the better competitors, especially in the cases of a disturbance-mediated creation of gaps in the landscape.
Based on the known functions of Anx2, we propose that the most likely mechanism behind our results is Anx2-mediated creation and/or stabilization of the PtdIns(4,5 P2-containing lipid raft membrane domains from which the virus preferentially buds [16]–[21].
We additionally tested the possibility that polyadenylation-mediated creation of termination codons occurs preferentially in certain functional gene categories.
Our analyses of published genomic data from Blastocystis sp. subtype 7 revealed that polyadenylation-mediated creation of termination codons occurs in approximately 15% of all nuclear genes.
Based on the foregoing estimate of approximately 15%, we assume that approximately 900 of the 6,000 annotated genes in this Blastocystis subtype rely on polyadenylation-mediated creation of termination codons.
C to U RNA editing-mediated creation of the AUG initiation codon from the original ACG codon is a common phenomenon observed in case of chloroplast genome-derived transcripts.
Genes exhibiting polyadenylation-mediated creation of termination codons in the Blastocystis sp. subtype 1 were identified in a draft genome assembly obtained from 454 and Illumina reads (Gentekaki E, Curtis B, Roger AJ, unpublished data) using EST data available for this isolate (Stechmann et al. 2008).
Such coordination tightly couples PolB1-mediated flap creation to Fen1-mediated flap cleavage.
As Blastocystis is a genetically diverse genus (Clark et al. 2013), it is of interest to know whether polyadenylation-mediated termination codon creation in nuclear mRNA transcripts is specific to subtype 7 or whether it is more widespread.
Indeed, we found a number of cases of poly(A -mediated terminA -mediated createrminationample of whicodon portrayed in figure 2. In this creationtriplet TCG in the sequence of angexampleofing a homolog of the RPC8 subunit of the RNA polymerase III is changed in a corresponding transcript to an in-frame termination codon TAA.
While N. benthamiana provides a more amenable model system to elucidate the molecular mechanisms underlying SP/HrpZ-mediated BNYVV resistance, the creation of stable transformants of sugar beet, the natural host of BNYVV, might prove an efficient strategy to combat rhizomania.
