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Bartual, S. G. et al. Structural basis of PcsB-mediated cell separation in Streptococcus pneumoniae.
We demonstrated a practical application of specific lectin binding by detecting hPSCs within a differentiated cell population with lectin-mediated staining followed by fluorescence microscopy and flow cytometry, and by enriching and purging viable hPSCs from mixed cell populations using lectin-mediated cell separation.
MM patient cells were separated from BM samples by antibody-mediated positive selection using anti-CD138 mactivatedcellated cell separation microbeads (Miltenyi Biotech, Gladbach, Germany).
This was expected because the main changes in texture related to cell separation result from enzyme-mediated structural and compositional changes in the cell wall.
Kin3-mediated transport of these Don1-loaded endosomes is critical for cell separation in order to synchronize Cdc42-triggered secondary septum formation with the accumulation of vesicles involved in lysis of the fragmentation zone (Fig. 2C and D).
In the cytoplasm, BAP1 regulates Ca2+-signaling mediated cell death.
For cell separation AutoMACS Pro (Miltenyi Biotech) was used.
Irving Weissman uses cell separation techniques to isolate pure blood-forming stem cells in mice.
Stem cell separation for cell replacement treatment.
Viability after cell separation determined by dye exclusion was >99%.
Infiltrated NK cells, could be recovered by magnetic cell separation.
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