In this study, we developed a 3D-printed, non-positive Poisson's Ratio (NPPR) scaffold intended for future use in stretch-mediated cell differentiation applications, such as in muscle and tendon regeneration.
Cardiac and skeletal markers were altered during OT-mediated cell differentiation.
Furthermore, we demonstrate that a functional TrkB receptor is needed for RA-mediated cell differentiation, indeed silencing TrkB causes a failure in morphological and biochemical cell differentiation in SH-SY5Y and LAN-5 whereas no effects were observed in SK-N-BE cells that express a non-functional TrkB receptor.
It has been suggested that RREB1 is possibly involved in RAS/RAF-mediated cell differentiation and augments the RAS/RAF-mediated transcriptional response [ 31].
Rosmarinic acid from Rosmarinus officinalis exhibits a mimetic neurotrophic effect in PC12 cells by inducing ERK1/2-mediated cell differentiation and enhancing cholinergic activity [ 100].
As RA treatment has been described to activate CREB and HDAC8 expression inactivates CREB, we hypothesize that the combined treatment with RA and HDAC8 inhibitors might synergistically act on CREB-mediated cell differentiation.
RREB1 is known to relate to RAS-mediated cell differentiation [ 29] and SP1 and KLF4 both represent binding sites for KLF-family proteins, where KLF4 is known to drive the differentiation of immune cells [ 30].
We found that one of the identified in this study lncRNA transcript, termed HOXD-AS1, is induced by RA, regulated by PI3K/Akt pathway and controls genes implicated in RA-mediated cell differentiation and also angiogenesis and inflammation, the hallmarks of metastasized cancer [ 29].
The RAS/RAF/MEK/ERK pathway is the classical RAS-MAPK signaling pathway implicated in growth-factor mediated cell proliferation, differentiation, and death [ 16].
Another study performed in C2C12 cells demonstrated that mTOR mediated muscle cell differentiation did require its kinase activity [ 193].
Cdx-2 is intestine-specific transcription factor mediating the extracellular matrix-mediated intestinal cell differentiation and is associated with intestinal-type gastric cancer [ 15- 19].
