In the future, the cell sorting approach described here may be combined with microfluidic delivery to enable Ab-mediated capture of T-lymphocytes or other cell types followed by release of select cells for downstream gene expression studies or re-cultivation.
Others have shown that endothelial p110δ activity is involved in selectin-mediated capture of rolling leucocytes [ 12, 31], an important endothelial process during inflammation, and it is possible that induced p110δ is important in this biology.
This technique is based on the cerium chloride-mediated capture of liberated phosphate groups in the presence of a phosphatase substrate such as glycerophosphate or p-nitrophenylphosphate (p-NPP).
The findings presented here not only highlight a previously unknown function of A3ARs, but also shed extra light on an underappreciated and poorly understood behaviour of neutrophils (that is, cytoneme-mediated capture of bacteria) [ 11, 12].
We hypothesize that flow-mediated capture of HSPCs with P-selectin may represent a practical and more physiological alternative to HSPC isolation than the static immunological or density-mediated protocols currently used in the clinic.
P-selectin/PSGL-1-mediated rolling and α4β1-integrin/VCAM1-mediated capture of encephalitogenic T cells on the brain endothelium is followed by α4β1-integrin-VCAM1 and LFA-1 ICAM1/2-mediated LFA-1 ICAM1/2-mediatedLFA-1 ICAM1/2-mediated
Its preliminary implementation by Zhang and coworkers [ 19] was not fully exploited, since these authors focused on hybridization-mediated capture of the selected clones, via hybridization with biotinylated oligonucleotides, designed within HCDR3 regions.
Apoptotic internucleosomal DNA fragmentation was quantitatively assayed by antibody-mediated capture of cytoplasmic oligonucleosome-associated histone-DNA complexes (Cell Death Detection ELISA Plus kit; Roche Molecular Biochemicals, Mannheim, Germany).
Although the pathway initiated by clathrin-coated pits has received the most attention because of its importance in receptor-mediated capture of external ligands (Conner and Schmid, 2003), it has recently become clear that other endocytic pathways exist, which serve additional physiological functions.
