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To test the hypothesis that the OE derived activity is mediated by released soluble factor(s), we applied OECM, made with OE from E14 mice, to dissociated E14 OB cultures and analyzed the mitral/tufted cell dendritic morphology changes.
This result shows that depolarization mediated by released FFA from lipolysis is required for Opa1 cleavage.
Also, DCFDA fluorescence mediated by released of reactive oxygen species (ROS) by microglia was found to be comparable in G1 mTerc−/− and G3 mTerc−/− microglia (8 months) under control conditions and when stimulated with 40 n m ATP (Fig. S1G).
Therefore, we may speculate that bradykinin-stimulated fibroblasts trigger a "purinergic wave" mediated by released ATP and metabolites formation that can affect sensory afferent nerve endings localized in the vicinity, representing the first insights of a fibroblast-neuron communication unproved so far.
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Induction of neuronal death is mediated by release of cytochrome c (cyt c) from the mitochondria though a well-characterized increase in outer mitochondrial membrane permeability.
Since acetylcholine also increases ciliary beat frequency and particle transport speed via activation of muscarinic receptors [24], [25], we also explored the possibility that the increase in cilia-driven particle transport was mediated by release of acetylcholine from the airway epithelium and subsequent paracrine activation of muscarinic receptors.
The inflammation spontaneously resolves, perhaps mediated by release of the anti-inflammatory cytokine TGF-β.
It has been suggested that cancer prevention by nonsteroidal antiinflammatory drugs (NSAIDs) is mediated by release of AA [ 8].
Apoptosis induction was mediated by release of mitochondrial cytochrome c, increased Bax : Bcl-2 ratio, enhanced activities of caspase-3 and -9, and PARP-1 cleavage.
However, the intensity and speed of propagation of the calcium wave, mediated by release from intracellular stores, was reduced in Pax6+/- cells.
This might explain why the effects evoked by oxytocin on gastric and intestinal motility in rat are mediated by release of cholcystokinin (CCK) (which inhibits gastric emptying) and CCK receptors, and differ from oxytocin effects evoked in human [ 21, 22].
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