The observed effects are suggested to be mediated by phosphorylation of Smad2 at sites that are also phosphorylated in response to TGF-β but are different from the carboxy terminal sites targeted by TβR-I.
Dr. Greengard found that important signaling systems in his medium-spiny neurons were mediated by phosphorylation.
Further analysis revealed that p53 stabilization was not mediated by phosphorylation of Ser-15 and Ser-20 residues.
Met signaling is mediated by phosphorylation of two carboxy-terminal tyrosines, which act as docking sites for a number of intracellular mediators.
The response of HSC to TGFβ, leading to, e.g., induction of α2 (I) collagen expression, is mediated by phosphorylation of Smad2 and Smad3 and subsequent nuclear translocation of a Smad containing complex.
The HGF- and IL-6-induced cPLA2 phosphorylation was blocked by the inhibitors of p38 and p42/44 MAP kinases, protein kinase C, calmodulin kinase, and tyrosine kinase, showing that HGF- and IL-6-induced AA release and PG production are mediated by phosphorylation of cPLA2.
For the beta 2-adrenergic receptor (beta 2AR), the rapid processes include functional desensitization, mediated by phosphorylation of the receptor by the cAMP-dependent protein kinase and the beta-adrenergic receptor kinase, as well as a loss of hydrophilic ligand binding proposed to represent sequestration of receptors into a cellular compartment distinct from the plasma membrane.
We found that the interaction of APP-BP1 and APP is mediated by phosphorylation of APP at threonine 668.
This is mediated by phosphorylation at Thr286 through the activity of the Ras/Raf/MEK/ERK cascade and the F-box protein FBXW8, which is an E3 ligase.
In addition, binding of HES6 to HES1 seems to cause proteolytic degradation of the heterodimer, mediated by phosphorylation of a specific serine residue in HES6 [13], [13].
Induction of the hCRH gene by the PKA pathway is mediated by phosphorylation of CREB at serine residue 133 [10] [10].
