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The replication block of the RM plasmid by the temperature shift decreased its copy number, leading to a decrease in the number of RM molecules, which in turn caused R-mediated breakage of insufficiently methylated genomic sites (Figure 1A, B), leading to the cell death process.
Plasmid DNA protection of LPE against Feton-reaction mediated breakage was used in this study.
This indicates that the NIE and NIE2 fusion proteins were not perfectly controlled and that I-SceI-mediated breakage occurred even in the absence of the inducing 4OHT ligand.
A DNA deletion in RH is the consequence of a radiation-mediated breakage, which is then repaired by the DNA-damage repair mechanism.
The local structure of chromatin influences its susceptibility to Spo11-mediated breakage (Brachet et al. 2012).
The R-mediated breakage might cause the DNA rearrangement (Figure 1B (ii)) [24], [25].
In an alternative model, Vps4-mediated remodeling or breakage of the first ESCRT-III ring could generate a second, smaller ESCRT-III scission complex that migrates towards the constriction zone.
This template switching has been called microhomology-mediated breakage-induced replication (MMBIR) or Fork Stalling and Template Switching (FoSTeS).
This event is believed to cause breakage of the water-mediated hydrogen bond between them, the central event for channel opening according to spectroscopic measurements.
Suppression of ATR-mediated phosphorylation of WRN prevents proper accumulation of WRN in nuclear foci, causing breakage of stalled forks [ 10].
Episodes of retraction terminate with release or breakage of the Tfp tether.
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