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The V3C-type and V3L-type Abs generated by the vaccinations were able to mediate virus neutralization.
Towards the development of such vaccines and other immune therapies, our studies are designed to identify regions of influenza viruses that are conserved and that mediate virus neutralization.
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This is significant, as protection is believed to be a result of antibody mediated virus neutralization [48], [49].
Numerous recent studies have shown the role of stalk-specific antibodies in neutralization of influenza viruses; the finding that stalk antibodies can effectively neutralize virus alters the existing dogma that influenza virus neutralization is mediated solely by antibodies that react with the globular head of the viral hemagglutinin.
For example, HSV-1 glycoprotein gC prevents complement-mediated cell lysis and virus neutralization [10], [11].
In neutralization assays, non-neutralizing 240C disrupted virus neutralization by mAb 341C and/or mAb 540C, whereas a combination of mAbs 341C and 540C blocked virus infectivity synergistically.
Sera were tested for BTV-4 neutralizing antibodies by Virus Neutralization Test (VNT).
Virus neutralization assays are designed to measure a reduction in virus infectious titer mediated by exposure to antibody.
Virus neutralization by sera samples was tested by a standard TZM-bl based assay.
The ELISA (Fig. 3) and virus neutralization raw data (Fig. 4) are readily available upon request.
In addition, serum and mucosal antibodies showed virus neutralization activity.
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