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Our results suggest that although habitat fragmentation and habitat loss are relatively low in the studied villages, economic change and, to a lesser extent, cultural change mediate transformations in the landscape.
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Jackstadt, R., Jung, P. & Hermeking, H. AP4 directly downregulates p16 and p21 to suppress senescence and mediate transformation.
For example, Scrib-deficient mutants cooperate with oncogenes to mediate transformation in Drosophila.
Agrobacterium-mediated transformation was used to transform soybean somatic embryos.
Arabidopsis protoplasts were transformed using PEG mediated transformation procedure and assayed for fluorescence by CLSM after 20 h [61].
Utilizing Agrobacterium-mediated transformation, we generated 78 primary transformants for LeTGA1 knockout and 130 primary transformants for SOLly GLB1 knockout.
Plants were transformed via Agrobacterium tumefaciens-mediated transformation [ 19].
S. cerevisiae INVSc1 (Thermo Fisher Scientific) was transformed using a lithium acetate-mediated transformation procedure38.
Nicotiana benthamiana transgenic lines producing Rs-cps dsRNA were generated from transformed callus tissues by Agrobacterium-mediated transformation.
The six different modified 35S constructs were introduced through Agrobacterium-mediated transformation and 194 lines of independent transformants were obtained.
These results suggest that the Cas9 gene fragmented during biolistic-mediated transformation, but not upon Agrobacterium-mediated transformation.
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