The CMV promoter has been reported to mediate strong expression in various cellular systems but its activity in mouse and human ESCs remains controversial [7] [9].
In three volvocine species, i.e., C. reinhardtii, G. pectorale and E. elegans, the PSAD promoter was shown to mediate strong expression [ 11, 20, 27].
Additionally, we used the MtPt4 promoter, which mediates a strong expression in mycorrhizal roots [ 15] for driving the expression of an amiR against a previously identified putative transcription factor (MtErf1).
The latter is mediating a particular strong expression in arbuscule-containing cells [ 15].
Arêde, P., Ministro, J. & Oliveira, D. C. Redefining the role of the β-lactamase locus in methicillin-resistant Staphylococcus aureus: β-lactamase regulators disrupt the MecI-mediated strong repression on mecA and optimize the phenotypic expression of resistance in strains with constitutive mecA expression.
Among the potential therapeutic viruses, alphaviral vectors are good candidates for cancer therapy because of the high level of transgene expression and their ability to mediate strong cytotoxic effects through the induction of p53-independent apoptosis [ 5, 6].
Therefore, it is reasonable to suppose that a process of signal transduction mediated by the act of wounding had happened in both areas, before the strong expression of the sesquiterpene synthase genes became evident at the early period of agarwood formation.
In addition, radiation induced strong expression of EDA2R, a member of the TNF receptor family that mediates p53-regulated anoikis [ 23].
The recombinant plasmid pCAMBIA1300-hph-PsCT pCAMBIA1300-hph-PsCT pCAMBIA1300-hph-PsCT containingd and transtrongd into T. rexpressionoptimized Agrobacassettemediated transformation, producing 324 positive T. reesei transformants for the twassteps of sconstructed
(E ) Adenovirus-mediated expression of myc-Munc18-1 in E2T packaging cells analyzed by SDS-PAGE and western blotting shows strong expression of recombinant protein (myc-Munc18-1, 67 kDa, actin, 43 kDa).
The new recombinant Ad5.TRP-1 mediated strong TRP-1 expression in infected HEK293T cells as confirmed by immunofluorescence microscopy using the TRP-1-specific monoclonal antibody TA99 (data not shown).
