Indeed, Ire1α mediated splicing of XBP1u mRNA was substantially reduced in Sec61α depleted cells during ER stress.
Upon activation of UPR, inositol-requiring protein 1 (IRE1α), the conserved ER stress sensor from yeasts to mammals, mediates splicing of the mRNA encoding X-box binding protein 1 (XBP1).
The two pathways can be functionally dissociated [ 47, 48], which is consistent with the fact that IRE1α autophosphorylation status in U87 cells does not strictly correlated with the IRE1 RNase-mediated splicing of pre-XBP1 mRNA [ 22].
Furthermore, CPP-PNA mediate splicing modulation is of considerable interest, since CPP delivery in contrast to lipofectamine mediated delivery holds some promise for in vivo and eventually therapeutic use [ 30, 31].
Regions of the C. virginica mitochondrial genome denoted as non-coding regions were screened for introns that might mediate the splicing of the discontinuous LSU rRNA gene.
Thus far, we have little understanding of how heteroallelic Csd proteins mediate the splicing of female fem messenger RNAs (mRNAs) or how Fem proteins direct the splicing of honeybee dsx (Am-dsx) pre-mRNAs.
Unbound IRE1 oligomerizes, autophosphorylates, and activates its endoribonuclease which mediates unconventional splicing of an intron from X-box-binding protein 1 (xbp1) messenger RNA (mRNA) producing a potent transcription factor (spliced-XBP1) [1] (Fig. 1).
Given the observed epistasis between alleles of cdc40Δ and rad6Δ after MMS treatment [54], and the failure of the correctly spliced ANC1 transcript to complement a cdc40Δ mutant's damage sensitivity [53], it is worth exploring whether Cdc40 mediates the splicing of the MMS2 and/or UBC13 transcripts as well.
In response to ER stress, the endonuclease activity of IRE1 mediates the splicing of bZIP60 mRNA to generate an alternatively spliced transcript that lacks transmembrane domain-encoding sequences.
