Essential features of the 5'-CS are the integrase gene (intI1), the product of which mediates orientation specific integration and excision of gene cassettes [6], a promoter region, containing the potential promoter, Pc to allow expression of the inserted cassette genes [7], and an integration site (attI1) for the site-specific insertion of gene cassettes [8].
The Saccharomyces cerevisiae monopolin complex directs proper chromosome segregation in meiosis I by mediating co-orientation of sister kinetochores on the meiosis I spindle.
mDia mediates Rho-regulated formation and orientation of stable microtubules.
In turn, the cellular phenomonenon mediated by this complex – regular orientation of the plane of cell division relative adjacent cells – allows the development and maintenance of organized, differentiated tissues, and this phenomenon in turn makes possible a higher level of biological complexity– the multicellular organism – from a collection of individual cells.
A structural polymorphism with two configurations is known to affect the orientation of LCRs that mediate the formation of 16p12.1 deletions (23, 24).
Moreover the proper orientation of newly formed tissue is mediated by pores through a process called contact guidance.
Natural killer (NK) cells are innate lymphoid cells which mediate resistance against pathogens and contribute to the activation and orientation of adaptive immune responses2,3,4.
If monopolin does not mediate co-orientation outside of a small budding yeast clade, then what does?
Additionally, at high concentrations of chloroform in the film, a perpendicular orientation of cylinders was observed where the solvent was able to mediate interfacial interactions sufficiently to prevent a preferential segregation of one of the blocks to the surface.
The orientation of the two helices and their hydrophobic interactions is mainly mediated by leucine residues and resembles a leucine zipper interaction, consistent with the predicted CC propensity of STIL-CC (Stevens et al., 2010).
