Since Rnf2 lacks DNA-specific binding activity, other factors must mediate its site-specific chromatin recruitment.
Therefore the 5' phosphate at the nick site may mediate its effect on cleavage site position at the level of the gapped duplex rather than the nicked X-junction.
The tyrosine kinases TrkC [ 39] and Src [ 40] also interact with BMPRII and could thus facilitate or mediate its tyrosine phosphorylation at sites required for the interaction to p55γ.
Finally, WHSC1 is phosphorylated in response to DNA double-strand breaks (Matsuoka et al., 2007; Pei et al., 2011), which mediates its recruitment to sites of DNA damage where it dimethylates H4K20 to recruit 53BP1 and promote DNA repair (Hajdu et al., 2011; Pei et al., 2011).
The tumour suppressor BRCA1 functions in homologous recombination repair to activate CtIP through ubiquitylation (Yu et al, 2006), which mediates its recruitment to sites of damage in cell cycle-regulated manner (Yun & Hiom, 2009).
They suggested that Kidins220/ARMS may target Trio to specific membrane sites, where it mediates its activity and induces local Rac1 activation.
This data indicates that the SBE of PAX3 promoter at −60 to −51 from the transcriptional start site mediates its transactivation by STAT3.
EEA1 does not contain any identifiable UIMs, but it might have a cryptic ubiquitin binding site that mediates its own ubiquitination.
Ultra-low-dose NLX co-treatment suppresses opioid tolerance and dependence by preventing these MOR signaling alterations [5], and we recently identified the NLX binding site that mediates its protective effects as a pentapeptide segment in c-terminal FLNA [9].
This inferred mutual exclusive relation is consistent with studies reporting that common sites along paxillin mediate its interactions with FAK and vinculin (Turner and Miller, 1994; Brown et al., 1996).
