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The LRR domains of FLRT3, which are essential for Unc5 binding, also mediate cell sorting of FLRT3 expressing cells [3].
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Since PAPC mediates cell sorting by down-regulating C-cadherin adhesion activity, we asked whether FLRT3 also mediates cell sorting.
A clue came from the observation that FLRT3 only mediates cell sorting activity when expressed at low levels that do not cause cell dissociation (Figure 1B and 1C).
Therefore, FLRT3 appears to be a more potent C-cadherin inhibitor than PAPC, and at low expression levels that modulate adhesion without disrupting tissue integrity FLRT3 mediates cell sorting, similar to PAPC.
As control, PAPC-TMC, an extracellular domain deleted PAPC mutant that is inactive in regulating C-cadherin mediated adhesion or inducing cell sorting [1], [9] (also see Figure 3A, 100 pg PAPC-TMC), did not show the cooperation with FLRT3 in inducing cell sorting like FL-PAPC and M-PAPC (Figure 3A).
fluorescent activated cell sorting.
magnetic activated cell sorting.
Fluorescence-Activated Cell Sorting.
d Fluorescence activated cell sorting (FACS).
TS performed sterile cell sorting.
SK participated in the cell sorting.
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