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We analyzed the raw signal intensities of probe sets using the standard Affymetrix strategy MAS5.0 and normalization by the global median scaling method.
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The intensities were also normalized across arrays using a median absolute deviation scaling method [ 22].
There are several methods for normalization including MAS5, Robust Multi-Array Analysis (RMA), and dChip for Affymetrix chips, median scaling for GE-CodeLink microarrays, and LOWLESS-based methods for cDNA two-color microarrays.
We also normalized the features of the music and humming data through mean-shifting, median and average filtering, and the min-max scaling method to eliminate the surrounding and peak noises that occur during recording.
Signal intensities from the chip were normalized by global median scaling, and copy number was assessed using several different algorithms (methods), relative to a reference model file generated from the 270 HapMap samples.
Then, the scaling method is applied to all the arrays to ensure that the medians of all arrays are equal.
We also tested two normalization methods that control for technical differences in intensity measurements between arrays and also between fluorescent dyes: Median scaling and eCADS (Grant et al. 2007; Dabney and Storey 2007).
The scaling method was set to "none".
The extracted pitch values are normalized by the mean-shifting, median filtering, average filtering, and min-max scaling methods.
All data points were median scaled to 1 using the median signal intensity value for data points labeled as present.
For each sample, the four biological replica raw median probe intensities were quantile normalized and median scaled to 50 [66].
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