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Both assays gave a positive reaction for all pathogenic Leptospira spp., with a median quantification cycle (Cq) of 16.43 (IQR 16.30 16.77) and 15.62 (IQR = 15.18 16.34) for the rrs and lipL32 assay, respectively.
Median quantification levels of all analyzed microbes were obtained from triplicate real-time PCR measurements.
The median quantification accuracy below 30% could be obtained among the three biological replicates of the femoral bone defect as well as for the soft tissue defect.
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Across all data points, the median relative quantification error of 90Y PET was +1.38% (mean +2.18%, 95%CI-3.92%to to +8.28%).
The arithmetic mean (with the standard deviation) and median relative quantification of THADA gene expression in thyroid normal tissue, hyper- and neoplasias are listed.
Median limit of quantification (LOQ) for placentas corresponding to a signal to noise ratio of 3 1, was 0.006 ng/g fat (range, 0.004 0.14 ng/g fat).
Median limit of quantification (LOQ) of a single congener was 1.3 pg/g (range: 0.06 14 pg/g) for tetra- to heptachlorinated and 8.8 pg/g (range: 1.1 36 pg/g) for octachlorinated PCDD/F congeners.
Two negative RT-qPCR controls were run on each sample plate, and the median cycle of quantification (Cq) value from each duplicate was used for analysis.>>> -wrap-foot>> -wrAllfoot> All data are expressed as means ± SEM, except gender.
However, quantification of median fluorescence intensity of CD11b and F4.80 revealed a trend towards reduced CD11b cell surface protein levels (median fluorescence db, 793±85 versus non-db, 1005±165; P=0.12) and significantly reduced F4.80 cell surface protein levels (db median fluorescence, 22±7 versus non-db, 53±7; P<0.01).
Indeed, quantification of median fluorescence in flow cytometry showed a 38% decrease in surface labeling compared to vehicle-treated control samples.
On quantification, the median level of methylation present was 0.0015% (range 0 to 1.3%), with only one sample demonstrating a level of methylation above 1%.
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