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Data values for each sample were normalized between the two platforms against the median of normal breast SAGE libraries, and Log2-based expression ratios were obtained.
Drier et al. proposed a personal pathway deregulation score (PDS) representing the distance of a single cancer sample from median of normal samples on the principal curve [ 5].
Expression changes in individual NT and T samples vs the median of normal liver are shown in Figures 1B and C, respectively.
Using published normative data, patients in this category (n = 3) exhibited less than the median of normal controls by 2 standard deviation (SD) in 2 or more of areas of cognitive function without significant clinical impairment [ 13].
Drier et al. (2013) proposed a personal pathway deregulation score (PDS), which represents the distance of a single cancer sample from the median of normal samples on the principal curve.
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Median percentage of normal glomeruli was 31% (range, 0%-1000%-100% median percentage of glomeruli with active lesions was 19% (range, 0%-1000%-100%
All chips were then normalized to the respective median values of normal, age-matched controls, as previously described [ 18].
Five expression profiles were identified: miR-654-3p miR-654-3p miR-654-3ptivexpression8b and mirepresentativeof in A, miR-18bandxpression representative of miR-18a in B, miR-92a1 expression in C, miR-155 expression in D And miR-146a expression in E. Forepresentativehe mRNA levels were nofmalized such that the median value of normal cells was of 1 (mean ± SEM, n = 3).
Five expression profiles were identified: miR-26a1 expression representative of let-7b, miR-101-1, miR-30c2, miR-143, miR-26b, miR-376c and let-7a1 in A, miR-30e expression in B, miR-193b expression in C, miR-342-5p expressinn in D and miR-190b expression in E. For each time, the mRNA levels were normalized such that the median value of normal cells was of 1 (mean ± SEM, n = 3).
Comparing patients in the subgroup that developed ESRD (n = 40) with those who did not (n = 50), second biopsy specimens from those reaching ESRD showed a higher median index of chronic damage (66.5% vs 29.0%) and decreased median percentage of normal glomeruli (6.0% vs 20.0%).
The median expression level in breast tumour tissue showed an IRS of 3 while the median IRS of normal tissue was 0. We next correlated nuclear FOXM1 expression with patients' survival data.
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