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The resting motor threshold (RMT) was determined using the method of Mills and Nithi [ 45]: the RMT was defined for each participant as the median intensity between the upper and lower threshold values.
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Following normalization and smoothing each probe's M value represents the median intensity difference between Cy5 and Cy3 of a 600 bp window.
Following normalization each adjacent ≥3 probe set value represents the median intensity difference between FUL, SCA, PAR and CRA and control FOR of a 600-bp window.
Median intensity was taken between technical replicates after checking pairwise Pearson correlation coefficients (r ≥ 0.98).
This will take headache score at treatment time into account (headache score varies from 0 to 6 with a median headache score at 4)), difference in median peak headache score, difference in median headache intensity between the two treatments at 4 h, AUC 0-2 h after sumatriptan/placebo and accompanying symptoms as nausea, photo- and photophobia.
To scale between arrays, the global median intensity per array was set at 1000.
Spots where the difference between spot and background median intensity was below half the background intensity for both dyes were removed from the analysis.
The median intensity of the treated bone tissue was compared between groups.
Histogram spot quantitation, known to give the lowest between-slide variability [ 48], was employed and median intensity values were used for subsequent analysis.
Overall, the median intensity difference of a single substitution at position 18 can vary between -4% and -8% in yeast depending on its effect on GC content.
Centers 2 has the highest median intensity.
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