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Based on paired read mapping, the libraries showed median insert sizes of 170 bp (PE), 3 kb, 5 kb, 8 kb, 15 kb, 20 kb, and 25 kb.
For each species, ~20 million paired-end sequence reads of 100 bp were generated from sequencing libraries with median insert sizes of 760 bp to 910 bp (Additional File 1: Table S1).
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Median insert size was 200 bp.
The median insert size between paired-end reads ranged from 131 to 151 bases per sample.
The median insert size, measured after removal of nonjumping and chimeric outliers, was 35.7 kbp.
Median insert size of PE data was 335 (with a median absolute deviation of 21), whereas median insert size of MP data was 2473 (with a median absolute deviation of 704).
On the Illumina platform, we generated 102,278,116 paired 79-mers with a median insert size of 340bp.
We constructed a DNA library with a median insert size of 300 bp for a paired-end read format.
Our simulated data had a read length of 100 bp and a median insert size of 200 bp.
DELLY hereby focuses on uniquely mapping paired-ends and the default insert size cutoff is three standard deviations from the median insert size.
RNA was chemically fragmented, using reagents supplied in the TruSeq kit, resulting in libraries with a median insert size of 155 bp.
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