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Median fold values greater than 1.0 indicated enrichment.
The median fold values (log2 FC (hrPC12/wtPC12)) of the possible REST target genes downregulated by REST only and the values of the possible nontarget genes downregulated by PRC only and by unknown mechanisms were all very close (−3.7, −3.8, and −3.7 ratios).
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The Affymetrix "present" tagged probe sets were ranked by the median fold change values between wild type and Cln1/5 knock out mice to "upregulated" and "downregulated" vectors.
After imposing the threshold, all fold-change values were centered by subtracting the median fold-change value of the 'no-site' mRNAs in each sRNA perturbation experiment, except in the case of Figure 5 figure supplement 1B,C, in which data were mean-centered.
In terms of calculated RNA-Seq median fold ratios, the values for all upregulated subfamilies were similar, with median close to 4, except for the REST only target genes that exhibited a ratio only slightly and nonsignificantly lower than the others).
The dotted lines indicate the median fold-change value for each distribution, otherwise as in Figure 1A.
Proteins with overall P-values <0.1, P-values of t-test <0.05 and P-values of median fold test <0.05 were selected as DEPs by the depletion of CB.
The cut-off for dichotomisation of gene expression values was the median fold change calculated for every gene.
The results were expressed as median fold (i.e., the ratio between the median fluorescence intensity values obtained in the presence and in the absence of treatment, respectively).
AQP: aquaporin; AC: adenocarcinoma; SCC: squamous cell carcinoma; fold change was calculated across microarray datasets (median); fold change in bold and marked indicates statistical significance (p-value < 0.05).
The relative fold change for each variable in turn is calculated, and all values for a spectrum are then divided by the median fold change for that spectrum.
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