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Median expression data for 207 GBM samples from two different microarray platforms combined was downloaded from the TCGA data portal.
We first used the median expression data of the genes in the eight samples to compute pairwise correlation coefficients in the R statistical environment, resulting in a correlation matrix of 12353 × 12353.
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Additional File 7 shows the processed (logarithmically transformed median expression ratios) data used for plotting cluster graphs for irradiated and bystander treatments.
For class prediction, log2 median centred expression data were analyzed using BRB-ArrayTools Version: 3.8.0-Beta_1 3.8.0-Beta_1il 2009) (http://linus.nci.nih.govBReleaseyTools.html).
Hierarchical cluster analysis was performed on these data with Cluster 3.0 using median-centered expression data by centered correlation and complete linkage clustering [ 35].
Kaplan Meier method was used to estimate PFS and OS probabilities for average gene expression data categorised at the median as low (⩽ median) vs high (> median).
For the organ and tissue transcriptome data, the median expression value within each data set was subtracted for each probe.
Median intensity gene expression data without background subtraction were normalized by a global lowess method followed by a print-tip median method with a modified version of the Goulphar script version 1.1.2 [ 54].
To investigate the association between CIS gene expression and survival, log-rank (Mantel-Cox) tests were performed on median split microarray expression data from human tumours [ 35].
(B ) Median RNA-seq expression data (Lott et al., 2011 ) of the three pre-MBT groups and the two MBT groups shows that paused genes are expressed at lower levels and tend to be induced later.
Median-subtracted arcsinh expression data from 20 different chicken tissues were taken from Chan et al. (2009).
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