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Effective doses (ED) values were calculated with Calcusyn software using the median effect principle defined by Chou and Talalay [ 26].
To determine the nature of combination of the two drugs, the median effect principle outlined by Chou [ 55] was utilized.
The effect of drug combinations was calculated according to the median effect principle described by Chou and Talalay (1984).
Note that the linear correlation coefficient, r, describes the conformity of the data to the median effect principle.
To determine whether the combination treatment is synergistic, the combination index (CI) was calculated using CalcuSyn software (Biosoft, Cambridge, UK) that uses the median effect principle.
The effects on cell growth were measured in cell based assays using the fixed molar ratio design and the median effect principle.
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Empirical methods such as isobolograms, median-effect principle and response surface methodology (RSM) are based on statistical experimental design and regression of data.
Combined-effects analyses were conducted according to the median-effect principle.
Drug concentrations inhibiting cell growth by 50% (IC50) were calculated from relative survival curves using the median-effect principle [ 16].
Synergism or antagonism after drug treatments was quantitated with the median-effect principle, using the combination index (CI) method [ 12].
Drug concentrations inhibiting cell growth by 50% (IC50) were calculated from relative survival curves (see Figure 2) using the median-effect principle [ 16].
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