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The cut-off value for the definition of high/low expression subgroups was the median density value; 77.75 was defined as the cut-off for peritumor tissues and 34.38 was defined as the cut-off for tumor tissues.
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Median density values and background values of each spot were extracted for both the experimental samples (Cy5) and the reference samples (Cy3).
Average SNP densities around miRNA target sites were computed at each position relative to the seed's 5′ ends, and reported SNP density values were estimated by the median density at positions 2 7 for the seed and the median density at 50 bp up- and down-stream of the seed for the flanks (excluding seed positions 1 8).
Background correction was done by subtracting local background median density from signal median density.
Background corrected signals were divided by their median on the array to obtain the normalized median densities representing the normalized expression values.
The results are given as the median ± interquartile range of the optical density values (450 nm) of each group.
Figure 1A shows optical density values for SIC and DRS antibodies, median and quartiles for control 1 and PSGN cases.
Zero was the median and mode of the distribution of total swine density and 149 was the 25th percentile of the distribution of observations with non-zero total swine density values.
Replicate density values were averaged.
The results indicated that physical access to such stores is positively associated with population density, median housing value, education, non-Hispanic Blacks, and Hispanics, and is negatively associated with median housing age.
Quantitative IHC analysis revealed that the median optical density (MOD) values of PTOV1 staining in all breast cancer were higher than that in control normal tissues.
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