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Overview of median coverage and the percentage with > 10× and 30× coverage is provided in Supplementary Table S1.
Figure 5A shows median coverage and GC content of each gene sorted with highest coverage on the left.
To overcome this limitation, one can invoke a proportionality between mean (or median) coverage and the underlying copy number, as assumed elsewhere [ 4- 6].
The bars in Figure 6 show the relative proportion of high coverage contigs (greater than median coverage), low coverage contigs (less than median coverage), and singletons for each area in the Venn diagram.
Given that coverage is modeled as a Poisson distribution, the variance for the median coverage can be approximated after adapting Raikov's theorem using the equation: (2) where c e is the expected segment median coverage and n' is the number of independent data points in the region (See Supplementary Material S.5).
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The output format for counts and coverage information across reference sequences is a simple tab separated file with fields for the reference sequence, annotation start/stop coordinates, counts, median coverage, mean coverage, and coverage standard deviation (see Additional file 1 for a detailed example).
Whereas in their non-amplified parts, their median coverage (13 and 14X, respectively was slightly lower than the average genome coverage (16X), it was much higher in the amplified part (21X).
The mean and median coverage were 111.9× and 65.9×, respectively.
Three transcriptomes were assembled from quality filtered sequences into 10,398, 6,882 and 7,195 transcripts, with a median length of approximately 700 bp and median coverage of 4.2, 4.7 and 4.7 for S. lineatus, S. mimosarum and S. tentoriicola respectively (see Table 1 for original and assembled data).
Single-cell genome libraries were sequenced to an average depth of ∼40X with 454-FLX, but the representation of specific loci ranged from 0 to >2,500X, with a median coverage of 8.6X and 7.5X for SAGs A and B, respectively (Table S2).
Freebayes (arXiv 1207.3907) was used to call SNPs in the mapping of male and female genomic reads versus Muller C exons (median coverage of 45 and 62 respectively).
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