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Fig. 3 Effect of ferulic acid on the median cell length Euglena gracilis under different light conditions.
The variation in median cell length after the addition of ferulic acid is shown in Fig. 3.
Because these lack a rigid cellulose wall and their pellicle is flexible, the cell shape would become round, resulting in a decrease in the median cell length.
The increase in paramylon content in the negative control group was likely due to a stress response, resulting in round cells with a shorter median cell length (Fig. 3) as well as slow motion and loss of vitality.
Cell length at hour 6 was longer than that of the other three periods, and the median cell length decreased from 22 μm in the control group to 19 μm in the negative control group but increased to 24 μm in the 500 mg/L ferulic acid treatment group.
The median cell length of the negative control group and the 10 mg/L ferulic acid treatment group decreased compared with that of the control group, but at higher concentrations of ferulic acid, cell morphology was restored to normal and the median length was longer than that of the control group.
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These images were processed using MATLAB (R2008a, The MathWorks) to determine median cell lengths and areas.
HID13 was chosen, from a range of other HI strains, as it showed flagellar motility, a median typical cell length for an HI culture, proportion of elongated cells, and retained predatory ability as determined by the YPSC overlay technique.
For this age group, we estimated a median cell-cycle length of ~9.8 hr, with median G1, S-phase, and G2 lengths of <30 min, ~6.8 hr, and ~2.3 hr, respectively, but with a long-tailed distribution of G2 lengths.
from the mean cell length.
Cell length, cell growth and cell division.
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