Sentence examples for median background values from inspiring English sources

Normalization of three biological replicates was performed using median signal values and median background values.

Median background values at 635 nm were subtracted from median spot values and the same was repeated for background and spot values at 532 nm.

The background adjustment was performed by subtracting median background values from the median expression values obtained by FE and then converting the results to log-scale.

When not directly provided, expression value was calculated as the median intensity value of a microarray feature minus the median background value.

Raw data were converted into cluster presence or absence by applying an expression threshold (set to 1.5X the median background value using a log2 scale).

A median background value was calculated around each of the 3757 features and subtracted from the mean feature signal to give the net signal for the respective gene.

The working signal intensities were generated using the mean foreground intensity values minus the median background intensity values as outputted from the GenePix Pro 5.0 results file.

For the gonad analysis median local background values were subtracted directly from median feature values, dye channels were swapped as appropriate and a 'printtiploess' normalisation (within slide) was applied.

Spot intensity values were calculated by subtracting the local median background from the median foreground value, and adjusting negative/zero values to 0.5 [74].

Median spot and individual median background (annulus setting) intensity values were extracted for each wavelength and imported into analysis software.

Median density values and background values of each spot were extracted for both the experimental samples (Cy5) and the reference samples (Cy3).