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However, in this report, C. saccharolyticus was grown in culture media with added yeast extract, which improves yields [ 22].
When grown on media with added hydrogen peroxide, the wild-type strain exhibits an increased catalase B activity compared to the control without oxidative stress.
Cultures of acetylene fermenters were grown on mineral salts media with added SL10 trace elements (Widdel et al., 1983) and vitamins (Oremland et al., 1994) with cysteine-HCl (SFB93) or Na2S (SV7) as reducing agent (Table 1).
Whilst phosphorus removal was consistently very high (typically around 85%), biofilter soil media with added organic matter reduced the phosphorus treatment effectiveness.
U87-MG cells were grown in MEM media with added 10%% FCS, 1 % glutamine, 1 % non-essential amino acids, and 1 % pyruvate.
However, only bioprecipitation was detected when the bacteria were grown in media with added organic matter.
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For cell treatment with 5-AzaC for a total of 36, 48 or 72 hrs, media with freshly added 5-AzaC was replaced at 24-hr interval.
Particular emphasis is laid on the choice of the artificial diffusivity parameters in order to sufficiently capture shocks and discontinuities in all the aforementioned continuum media with minimal added dissipation.
Interestingly, Bacillus anthracis was present and dominant in all six STM exposures (0 50 mg L−1), but the MIC results showed high sensitivity to STM (< 6 µg mL−1) and they were not grown in the growth agar media with low added STM dose (0.1 mg L−1).
In practical terms, the cells need anaerobic conditions to grow on solid media; however, they can grow in liquid media with oxygen added (data not shown) [ 41].
Cells were passaged into fresh media with selection added the next day (neomycin-G418, 1 mg/ml; Sigma-Aldrich, St Louis, MO).
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