Both LB and M9 media were supplemented (when required) with ampicillin to a final concentration of 100 μg/ml.
When required, media were supplemented with ampicillin (100 µg/ml), and tetracyclin (25 µg/ml).
When required, media was supplemented with chloramphenicol (50 µg/ml), erythromycin (250 µg/ml), kanamycin (50 µg/ml), or tetracycline (10 µg/ml).
When required, media were supplemented with chloramphenicol (Cm; 25µg/ml), tetracycline (Tet; 12.5 µg/ml), 2, 6-diaminopimelic acid (DAP; 50 µg/ml), L-arabinose (0.05% or 0.2% wt/vol), D-mannose (0.2% wt/vol), D-lactose (1% wt/vol), D-maltose (1% wt/vol), sucrose (5% wt/vol), L-cysteine-HCl (22 µg/ml), or L-tryptophan (20 µg/ml).
When required, media were supplemented with antibiotics: ampicillin (100 μg/ml), kanamycin (100 μg/ml) and chloramphenicol (25 μg/ml).
When required, media were supplemented with chloramphenicol, ampicillin, or nalidixic acid, at concentrations of 15, 50 and 20 mg/l, respectively.
When required, culture media were supplemented with ampicillin (100 μg/mL), chloramphenicol (30 μg/mL), erythromycin (50 μg/mL or 30 μg/mL for liquid cultures and plates, respectively).
When required, culture media were supplemented with antibiotics at the following concentrations: ampicillin, 100 µg ml−1; tetracycline, 20 µg ml−1; kanamycin, 50 µg ml−1 and gentamycin, 10 µg ml−1.
When required, the media were supplemented with 1.5% agar, 5% sucrose, colistin sulphate (Col; 12.5 µg/ml), ampicillin (Amp; 100 µg/ml), chloramphenicol (Cm; 25 µg/ml), or kanamycin (Km; 50 µg/ml).
