Sentence examples for media were washed from inspiring English sources

G-Rh2-treated cells in serum free media were washed with PBS, and cell pellets were re-suspended in lysis buffer containing 20 mmol/L Tris, pH 7.5, 0.5% Triton X-100, 2 mmol/L MgCl2, 1 mmol/L DTT, 1 mmol/L EGTA, 50 mmol/L β-glycerol phosphate, 25 mmol/L NaF, 1 mmol/L Na3VO4, 2 mg/mL leupeptin, 2 mg/mL pepstatin A, 2 mg/mL antipain, and 1 mmol/L PMSF for 1 h.

Experiments that required maintenance of cells in "stripped media," were washed with phosphate buffered saline, and then the media was changed to EMEM without phenol red that contained 10% charcoal dextran – treated FBS (CD-FBS).

Strains of Y. lipolytica grown on oleic acid containing media were washed three times with 0.5% BSA solution prior to application of the protocol for spheroplast and organelle preparation.

To prepare cells under a poor condition, the cells grown overnight in YPD media were washed with ddH2O, resuspended in 100 ml of SD (0.67%(w/v) yeast nitrogen base without amino acids/2%(w/v) glucose) at an OD600 of 0.5, and grown at 30°C for 6 hrs.

Therefore, to eliminate interference from tumor cytokines in the co-cultures, tumor cell conditioned media was washed away just prior to adding the monocytes.

At the two leaf stage, the media was washed off the roots and the germinating seedlings were transplanted into a 96-well tray containing sunshine mix.

The next day, culture media was washed out and ganglia were treated with five pulses of 5-HT, as previously described (Monje et al. 2012).

Repeat steps 6 and 7 to make sure all media is washed out of the cells.

The environmental perturbations were given in a pulse-like addition to the media, and were washed out of the media on a time scale of ∼5 hours (corresponding to the dilution rate).

After incubation with 100−1000 nM of each Cre fusion protein for 4 h in serum-free media, cells were washed to remove surface bound protein and incubated in full media for 48 h.

In experiments involving AIM V serum-free media, cells were washed extensively with sterile PBS, and then preincubated for 24 h at 37°C with AIM V-containing culture media to prevent contamination with serum.