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The two best performing media were then further tested to identify the underlying pollutant removal processes.
All media were then removed, and 100 μL of DMSO was added to each well.
The collected media were then ultracentrifuged at 100,000 × g for 1.5 h at 4 °C.
Cell culture media were then collected and concentrated using Amicon 10 K (Millipore).
The media were then collected for measurement of glucose and lactate concentration and cells harvested for protein lysates.
Some French media were then asked to "sell" the project of the "blue men of the desert" who were willing and ready to go to war against Mali.
UV radiation exposure was carried out in a Spectrolinker XL-1000 crosslinker with a minimal volume of media covering the cells during irradiation; fresh media were then added.
The media were then removed and cell monolayers were washed once with PBS, and then overlaid with 1.0 mL culture media containing 0.75% agarose.
Complete media were then added to the polystyrene dish at room temperature to inhibit the effects of trypsin.
The nanoparticles in aqueous micellar media were then subjected to UV-vis, SAXS, fluorescence, and TEM measurements.
The media were then discarded from the wells, and 200 μL dimethyl sulfoxide was added to solubilize the formazan crystals that had formed in the interim.
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