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The established reporter cells REPON5.4 and REPON8.3 were exposed to various pathophysiologic substances, and culture media were subjected to SEAP assay to identify regulators of nephrin gene expression.
Biotinylated and unmodified BAEC, cultured for 1 h in serum-containing media, were subjected to laminar flow in a variable-height flow chamber that provided a range of shear stresses from 0.2 to 75 dynes/cm2.
All publications and media were subjected to censorship.
The culture media were subjected to the concentration of murine IL-1β, IFN-β, and IL-12p70 by quantitative sandwich enzyme immunosorbent assay using a mouse specific ELISA kit (Biosource, Camarillo, CA) according to the manufacturer's instructions.
The same media were subjected to the concentration of mouse IL-6, MCP-1, TNF-α by a Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD Biosciences, San Diego, CA) using a FACScalibur and CellQuest software (Becton Dickinson, Tokyo, Japan).
NPC cell conditioned media were subjected to sequential centrifugations.
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The media are subjected to an external constant magnetic field and could be rested on a Winkler-type elastic foundation.
Furthermore, like their living counterparts, nano-objects and nanodevices moving in liquid media are subjected to viscous rather than inertial forces.
An engineered leak surrounded by porous media was subjected to a pressure transient resulting from the rapid closure of an upstream valve.
The conditioned media was subjected to fibrin and gelatin zymography as described by Gondi et al. [ 23].
Throughout much of the twentieth century, the electronic media were subject to congressionally and administratively mandated responsibilities to operate in the "public interest". In the current deregulatory climate, however, as the government has relaxed its "public interest" standards, this system does not provide the counterweight it once did.
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