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The media were sterilized by autoclaving while the pretreated liquor was sterile filtered (0.22 μm Stericup, Millipore, Billerica, United States).
All these media were sterilized for 20 min at 121 °C in a portable sterilizer.
The rest of the media were sterilized separately and allowed to cool down, and fresh inoculum was mixed with sterile media in a laminar air flow cabinet.
All media were sterilized by autoclaving at 121 °C for 15 min.
These media were sterilized for 20 min at 121 °C.
All media were sterilized by autoclaving at 121 °C.
Similar(29)
The media was sterilized through filtration (0.22 μM) before use.
A set of test tubes containing 1 mL culture media was sterilized in an autoclave at 15 psi pressure at 121 °C for 20 min.
Nutrient Broth media was sterilized in autoclave, and distributed equally to four flasks of 250 mL each.
All the glassware and media used were sterilized in an autoclave at 121 °C for 20 min.
All media types were sterilized by autoclaving at 121°C for 30 min.
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