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After 10 days of incubation, the cells and the media were separated by filtration.
When materials from the cell lysate and media were separated according to mol wt (Mr), at least three different pro-LHRH species were detected.
These media were separated by a 5 mm gap preventing glucose to diffuse into the medium without carbon source.
The 20 kDa and 40 kDa bands of KCNE2 from the cell culture media were separated on 4 15% SDS-PAGE and visualized on gel by silver staining method (Silver Stain Plus kit, Bio-Rad).
Cells (OD750 = 3.3 ± 0.3) and culture media were separated by centrifugation.
Afterwards, the media were separated from the tissue and applied to the cation-exchange columns (Dowex 50+W).
Similar(44)
The calculated results show that the dopant concentration strongly affects the radiation heat flux when the two media are separated at nanometer distances.
Frictionless contact (zero shear stresses and discontinuity of tangential displacements at the interface) is relevant, for instance, when media are separated by a thin fluid layer.
Blogs and mainstream media are separated.
Figure 1(b) shows that the scala vestibuli is at the top, which is separated from the scala media by a thin flexible partition called Reissner's membrane, and the scala media are separated from the scale tympani at the bottom by a rigid partition that includes a more flexible section called the basilar membrane.
But these media are separate and run to different rhythms.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com