Sentence examples for media were resolved from inspiring English sources
In short, 60 h after transfection of mock, pSV and pM, 3 mL of serum-free medium were added, and cells were incubated for another 12 h and the conditioned media were resolved over 0.1% gelatin-SDS-polyacrylamide gels.
Total cell lysate protein (5 μg) and concentrated culture media (an equivalent volume of 500 μL of non-concentrated culture media) were resolved by electrophoresis on 15% sodium dodecyl sulfate polyacrylamide gels (SDS PAGE) and probed with the primary anti-HA antibody (Roche, 3F10, 1 1000) and the secondary anti-rat IgG-(Fab)'2 HRP) antibody (Abcam, ab6517, 1 40 000).
The concentrated conditioned media samples were resolved on SDS-PAGE and immunoblotted for proteins of interest.
Equivalent amounts of protein from cell lysates or conditioned media (CM) from each treatment group were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted with primary antibodies.
"I'd prefer the issues were resolved in private without media and PR companies and press releases.
Those issues were resolved.
Her complaints were resolved).
Any discrepancies were resolved.
"Many of the issues that have dogged rich media have been resolved," Kliger says.
For western blot analysis of media protein, 40 μl conditioned media per sample was resolved and transferred as above, and blots were stained with Ponceau S to demonstrate equal loading and photographed.
