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Media were replenished every three days.
The following day, media were replenished and nanofibrous scaffolds were introduced.
Fresh IL-2 and fresh X-VIVO 15 media were replenished every 3 days.
The BHI media were replenished every 3 days while incubated (Shel Lab, Vernon Hills, IL) at 37 °C.
The different types of media were replenished every 24 hours.
The concentrated media were replenished with fresh α-MEM to the original volumes.
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HOKs were cultured until 80% confluent and the media was replenished every 3 4 days.
Media was replenished on the second day and aggregates were plated on plastic 10 cm dishes on the fourth day.
After plating epithelia into Matrigel stromal inserts were added to the cultures, media was replenished every 2 3 days.
For inhibition studies, LY-364947, AMD3100, PD173074 or vehicle (Sigma) were added from day 0, media was replenished every 2 3 days.
Media was replenished after 48 hours and 96 hours, and cell viability was assessed after seven days using CellTiter Glo Luminescent Cell Viability Assay (Promega, USA) as per manufacturer's instructions.
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