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Culture media were renewed every 2 days.
The culture media were renewed every two days.
Media were renewed every 2 3 days.
Every second day, media were renewed in presence of DMSO or 2 µM of SB431542.
For biofilm growth assays longer than 24 h, media were renewed every 24 h by placing the pegs in a new microtiter plate containing media.
The day of transfection, culture media were renewed and siRNA mixtures prepared using PrimeFect siRNA Transfection Reagent diluted 1/100 in PrimeFect diluent (purchased from Lonza) and incubated 15 minutes at room temperature before adding to cells.
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All the algal culture media was renewed on a weekly basis with bacterial-free seawater containing 0.1 mM KH2PO4 and 0.1 mM NaNO3.
The DNA solution was dropwise added to 150 µL HEBS (280 mM NaCl, 50 mM HEPES, 1.5 mM Na2HPO4, pH 7.1) under vigorous vortexing and incubated at room temperature for 10 min. After 12 h the transfection media was renewed and the virus containing supernatant was collected two and three days after transfection.
Culture media was renewed 24 h after application of siRNA.
The media was renewed after the first 24 h of incubation.
After 24 hours, the media was renewed and test treatments initiated.
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