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Media were removed after 48 h and 96 h and replaced with fresh media containing treatments described below.
Media were removed and the plate was washed with 1% FBS and norepinephrine-free media.
Culture media were removed from wells.
After 4 h, culture media were removed and cells were washed twice with PBS.
Then, culture media were removed and washed twice with 1 ml of PBS.
Culture media were removed, and 1 ml of acidic isopropanol was added to each well.
After the incubation, the media were removed, and each well was washed with fresh ice-cold media (2 × 2 mL).
After 2 and 4 days of incubation, the media were removed and the cells washed gently with PBS.
After cell culture experiments, culture media were removed and cells were washed two times with PBS at 37°C.
For cell density readings, suspended cells were pelleted and growth media were removed.
Culture media were removed 48 h after cells were infected with virus and treated with ATA, AH or both.
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