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Proteins in the conditioned media were precipitated using trichloroacetic acid (TCA) and separated by SDS-PAGE.
Two-hundred microliters of MTB culture media were precipitated and processed by on-chip fractionation, as described previously.
Equal volumes of conditioned media were precipitated with 80% ice-cold acetone and incubated at −20 °C for 1 h.
Cells from the hair follicle rich skin of P1 pups were cultivated in microvesicle depleted media (precleared by ultracentrifugation) and the conditioned media were precipitated at 120000 g for 90 min to collect microvesicles.
Proteins secreted by A. pleuropneumoniae into the culture media were precipitated by the addition of 0.02% deoxycholic acid (v/v) and 15% trichloroacetic acid (v/v) to the supernatant.
Similar(55)
Conditioned media proteins were precipitated with an equal volume of 12% TCA.
Proteins from BMDM media supernatants were precipitated using TCA plus 200 μg insulin carrier protein and separated on 13% gels.
Secreted proteins from media supernatants were precipitated with 0.1% sodium deoxycholate and 7% trichloroacetic acid, followed by washes in acetone and resuspension in phosphate-buffered saline (PBS).
In addition, in SM5 and SM6 solid media, only bassanite and gypsum were precipitated.
Single nanoparticles, which were precipitated in aqueous media, form aggregates, when the composites are in a contact with polar organic solvent.
To analyse zeta potential values of all components in this co-cultivation media, SD277 and A. fumigatus cells growing separately in monocultures were precipitated and resuspended in co-cultivation media.
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