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At the end of each culture period, the media were pooled to measure hormone levels, and the cultured follicles were collected to measure expression of cell cycle regulators and steroidogenic enzymes.
Four independent experiments were conducted and the conditioned media were pooled.
Additionally, the exponential and stationary phase RNA preparations each from GMX and TY media were pooled.
Fractions containing 70S particles in the WT strain grown in N M9 media were pooled and stored at −80°C for use as a reference sample.
Conditioned media were pooled from 24-hour and 48-hour serum-free incubations and HGF was quantified using a HGF-specific ELISA assay according to the manufacturer's instructions (R&D Systems, Minneapolis, MN, USA).
Finally, media were pooled, diluted appropriately and individually aliquoted (150 positive and 100 negative aliquots) to yield a positive independent control with an antigen value of (circa) 600 U l−1.
Similar(52)
Adherent cells were rinsed once with culture media and the liquid media is pooled with the non adherent fraction.
Media was pooled from all plates within each treatment, and stored at -20°C for melatonin RIA analysis.
To verify the conditioned media used was the same from each notochordal culture all media was pooled for NCT and NCA respectively.
Multiple explants were prepared from each fish, and the subsequent media for harvest were pooled per individual fish.
We preserved the culture media (insert and well were pooled) at –80°C for subsequent analysis of hormone production.
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