Sentence examples for media were plated from inspiring English sources
As transfection control served a non-silencing negative control (Ctrl_AllStars_1 (SI03650318); Qiagen). 5 × 105 (A204, BT16 and G401) cells in 500 μl antibiotic-free media were plated on 24-well dishes and transfected with siRNA at a concentration of 10 nM using 4.5 μl HiPerFect Transfection Reagent (Qiagen).
5 × 105 cells of BT16 cell line in 500 μl antibiotic-free media were plated on 24-well dishes and transfected with (1) siRNA for single knockdown of WWC1, NF2 or FRMD6 (10 nM) and the non-silencing negative control siRNA (20 nM), (2) a combination of all three siRNAs for triple knockdown of WWC1, FRMD6 and NF2 (10 nM each), (3) non-silencing siRNA (30 nM) as a negative control.
About 1×10ˆ5 HEK293 cells per well in 500 microliters of growth media were plated in 24-well plates one day prior to transfection.
Cells (104 cells in 100 µl of media) were plated into each well of a Greiner Bio-one tissue culture grade 96-well white clear bottom microassay plate (PBI S.p.a., Italy) and incubated in 5% CO2 atmosphere at 37°C for 24 hr.
Cells (1.0 × 10) suspended in serum-free media were plated on top of the Matrigel.
After 20 minutes, the same numbers of HepG2 cells in antibiotic free media were plated in each well and incubated at 37°C.
In Group 1, the cell-containing media was plated either at high (5000 cells/cm2) or low density (1000 cells/cm2).
An aliquot of the bottom chamber media was plated for bacterial counts and another was used to measure fluorescence emission at 520 nm in a LS 50B Luminescence Spectrometer (Perkin Elmer) to monitor the presence of FITC-dextran.
For the soft agar assay, a base layer of 1% (w/v) agarose/cell culture media was plated into six-well plates and allowed to solidify.
For migration assay using MEK1 inhibitor (PD98059, Millipore, Billerica, MA, USA), cells (1 × 10) along with 10 μℳ MEK1 inhibitor or media alone were plated onto the upper chamber of transwell chambers and media on the bottom chamber.
Beads were resuspended in NP 10% v/v serum-supplemented media and were plated into 12-well culture plates with approximately 10 to 15 beads per well.
