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Aliquots (190 μl) of the heparin-release media were mixed with 10 μl of the substrate and incubated at 37 °C for 1 h.
Samples (500 μL) of cell free culture media were mixed with 150 μL of D2O as internal lock and immediately analyzed.
For SILAC experiments, cells grown in heavy and light media were mixed in equal numbers and lysed by bead beating in a buffer containing 8 M urea, 75 mM NaCl, 50 mM Tris-Cl, pH 8.2, and a protease inhibitor cocktail (complete mini, Roche, Germany) using three cycles of 90 s separated by three minute incubation on ice.
As the news broke Thursday, fan reactions on social media were mixed: But least one of Young's colleagues has already expressed support, and has hinted that Young's sexuality wasn't a suprise.
Comments on social media were mixed - while many mocked PIA for indulging in superstition, others defended the slaughter as a practice endorsed by Islam.
The media were mixed fully and then sterilized at 120 °C for 30 min. 5 mL inoculum was inoculated into 100 mL medium in 250 mL flask.
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Media are mixed.
Reaction on social media was mixed.
When the same parts and media are mixed in a vibrating tub, the process becomes even more productive.
Briefly, 30 μl culture media was mixed with 100 μl acid phosphatase reagent.
Briefly, 30 μL culture media was mixed with 100 μL TRAP reagent.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com