Sentence examples for media were lysed from inspiring English sources

Cells grown in "light" media were lysed, incubated with the K4me3 probe, and subjected to UV irradiation.

1.5 mL of positive blood culture media was lysed with 1.5 mL of 5 M guanidinium-HCl-100 mM Tris (pH 8.0) [ 15].

Reactions were stopped by aspiration of media and cells were lysed in 50 µl of lysis buffer containing 1 mM 3-isobutyl-1-methylxanthine.

After 48 h incubation with cell culture media, then cells were lysed in passive lysis buffer (Promega, Wisconsin, USA).

Percent cytotoxicity was determined by the ratio of LDH activity in the media before lysing the cells to the LDH activity in the media after the cells were lysed.

At different time-points, the infected monolayers were washed three times with antibiotic free media, and the cells were lysed using 0.1% deoxychloate solution.

After collecting the conditioned media, also the cells were lysed to Laemmli buffer and separated on gel similarly.

The experiments were ended at various time points, depending upon the protocol, then media were collected, cells were lysed and the lysate was collected for quantitative PCR (qPCR) analysis.

The media was removed and cells were lysed in dimethyl sulfoxide, using an empty well as a blank.

Media was removed and cells were lysed in modified RIPA lysis buffer(Santa Cruz Biotech) supplemented with PMSF, Na3VO4, and 1∶10 protease inhibitor cocktail in 1X PBS made from tablets (14654600, Roche Diagnostics, Indianapolis, IN).

Conditioned media were harvested and cells were lysed as described elsewhere [ 24].